Composite

Part:BBa_M50033

Designed by: Cale Lester, Raquel Freeman, Tristan Yeung   Group: Stanford BIOE44 - S11   (2016-11-01)


U1_mTurquoise2, IPTG-induced, CAM resistant Thermosensor Construct

At elevated temperatures, the U1 thermosensor releases its hairpin structure and allows the RBS to be readily accessible to the ribosome, enabling increased expression of our desired gene. U1 has a unique trait that allows for the detection of increasing gene expression levels ranging from 22˚C to 37˚C. It contains a pP-T5 promoter, U1 RBS, mTurquoise2, and a pT-T7 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 61
    Illegal PstI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 61
    Illegal PstI site found at 67
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 49
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 61
    Illegal PstI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 61
    Illegal PstI site found at 67
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None